Gough Lab – Dept Chemistry – U Manitoba

Molecules can be identified by their energy absorption characteristics. Infrared wavelengths can be used for in situ chemical analysis based on their interactions with specific chemical bonds. We are using synchrotron FTIR spectroscopy to characterize maturation-related changes in Aspergillus nidulans hyphae at NSLS (Brookhaven, NY), SRC (U Wisconsin-Madison) and CLS (U Saskatchewan).

Photomicrographs and FTIR spectra acquired along hyphae of permissive (a) and restrictive (b) A. nidulans hypA1 phenotypes. Scale bars 50 µm. This image is from Szeghalmi, Kaminskyj and Gough,  A synchrotron FTIR microspectroscopy investigation of fungal hyphae grown under optimal and stressed conditions Analytical and Bioanalytical Chemistry DOI 10.1007/s00216-006-0850-2

The image below was collected at NSLS.

Pictured below, from left to right, are Dr. Susan Kaminskyj, Dr. Kathy Gough, and Konstantin Jilkine at CLS 01B1-1 (Mid IR) beam line, August 2007